Figure 6.
Blocking and knock-down studies. (Left) UMUC3 cell number in response to integrin blockade (Anti integrin α3, anti integrin α5), to CD44s blockade (Anti CD44), and to blockade with defactinib (Defactinib). Untreated cells served as controls. Cell number was evaluated after 24, 48, and 72 h by the MTT assay (n = 3). (Right) cell growth of UMUC3 treated with a CDK1-, CDK2-, or Cyclin A-specific siRNA, evaluated by the MTT assay. The Western blot on lower right depicts reduction in CDK1 and CDK2 by siRNA knock down (Supplementary Figure S1). One representative of three separate experiments is shown. All values are related to the untreated controls set to 100%. Mock indicates scrambled controls. * indicates significant difference to the untreated (unblocked) control or to cells not treated with siRNA.