Figure 6.
Pattern of Tissue and Cellular Expression of Sxd1 in Wild-Type and sxd1 Mutant Maize Plants.
(A) RNA gel blot showing levels of Sxd1 mRNA in wild-type (WT) and sxd1-1 tissues. Each lane contains 5 μg of total RNA collected from the indicated tissues. The lower panel shows 18S rRNA as a control for loading.
(B) In situ hydridization of maize leaf sections with Sxd1 probes. Transverse sections from a mature wild-type leaf hybridized with Sxd1 antisense (upper) and sense (middle) probes. The lower panel shows a section of a matched leaf from an sxd1 mutant plant hybridized with the Sxd1 antisense probe.
(C) Protein gel blots comparing protein expression in BS and mesophyll (MS) cells. Detection of SXD1, PEPCase, and RBCL is shown in two independent preparations of each cell type. PEPCase and RBCL distributions indicate the level of enrichment for each tissue, because PEPCase and RBCL are MS and BS specific, respectively.