Table 1.
Arabidopsis Growth Stages for the Plate-Based Phenotypic Analysis Platform
Col-0 Data
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Stage | Description | Daysa | sd | CVb |
Principal growth stage 0 | Seed germination | |||
0.10 | Seed imbibition | 3.0 | NAc | NA |
0.50 | Radicle emergence | 4.3 | 0.4 | 10.3 |
0.7 | Hypocotyl and cotyledon emergence | 5.5 | 0.6 | 11.2 |
Principal growth stage 1 | Leaf development | |||
1.0 | Cotyledons fully opened | 6.0 | 0.5 | 8.5 |
1.02 | 2 rosette leaves >1 mm | 10.3 | 0.6 | 5.8 |
1.04 | 4 rosette leaves >1 mm | 14.4 | 0.5 | 3.4 |
Stage R6 | More than 50% of the seedlings have primary roots ≥6 cm in length | NDd | ND | ND |
a Average day from date of sowing, including a 3-day stratification at 4°C to synchronize germination.
b CV, coefficient of variation, calculated as (sd/days) × 100.
c NA, not applicable.
d ND, not determined (see text for details).