Figure 7.
Transcript Levels for Cold-Regulated Transcription Factors RAV1, ZAT12, and RAP2.1.
(A) Two-week-old wild-type (Wassilewskija-2) plants grown at 22°C were cold treated at 4°C, and tissue was harvested at the times indicated. Total RNA was isolated, and RNA gel blots were prepared (10 μg of RNA). The blots were hybridized with 32P-labeled probes for RAV1, ZAT12, and RAP2.1.
(B) Total RNA was isolated from 2-week-old plants from transgenic lines expressing the indicated CBF genes under the control of the 35S promoter of Cauliflower mosaic virus or carrying the empty vector (V). Total RNA was isolated from plants grown at warm temperature, and RNA gel blots were prepared (10 μg) and hybridized with a 32P-labeled probe for RAP2.1.