Table 2.
Effect of tocopherols on nitrite accumulation and iNOS expression
| Treatment | Nitrite
release, μM
|
PGE2, ng/106
cells
|
iNOS expression,* % | ||
|---|---|---|---|---|---|
| Control | l-NMMA (1 mM) | Control | l-NMMA (1 mM) | ||
| LPS | 24.0 ± 3.1 | 2.1 ± 0.3† | 8.3 ± 1.1 | 8.4 ± 1.0 | 100 |
| γT (10 μM) | 19.4 ± 1.6* | 2.2 ± 0.2† | 3.5 ± 1.0** | 3.7 ± 0.8** | 69 ± 12** |
| αT (10 μM) | 22.1 ± 1.4 | ND | 7.8 ± 0.9 | ND | 76 ± 10* |
| γ-CEHC (10 μM) | 23.1 ± 2.6 | ND | 6.5 ± 0.6* | ND | 96 ± 8 |
RAW264.7 cells were preincubated with vehicle and various tocopherols for 14 h and then treated with 0.1 μg/ml LPS in the presence or absence (controls) of 1 mM NG-monomethyl-l-arginine (l-NMMA), for 14 h, at which time medium was collected and nitrite accumulation was measured. *, P < 0.05 and **, P < 0.01: vehicle vs. γT, αT, and γ-CEHC supplementation; †, P < 0.01: with vs. without 1 mM l-NMMA; ND, not determined.
*
iNOS (%) was obtained, using the Western blot, by the comparison of iNOS induced in the presence of various tocopherols with that expressed with ethanol alone.