Figure 6.
Gut granules are sites of fat storage in embryos. Nile Red– (B) and BODIPY493/503 (E)-stained, fat-containing compartments colocalized (white arrows) with birefringent gut granules in wild-type pretzel-stage (C and F) embryos. Pretzel stage pgp-2(kx48) and apt-7(tm920) embryos contain a significantly reduced number of Nile Red–stained compartments and mislocalize Nile Red–stained fat into the intestinal lumen (H and K). The Nile Red staining in pgp-2(kx48) and apt-7(tm920) intestinal cells colocalized with birefringent material (white arrows in H–I and K–L). The intestinal cells of glo-1(zu437) pretzel-stage embryos lack Nile Red–stained compartments and mislocalize Nile Red–stained fat into the intestinal lumen (N and O). In a 1.5-fold–stage embryo expressing the gut granule–associated protein GLO-1::GFP, Nile Red staining is contained within GLO-1::GFP marked organelles (P–R). Intestinal cells are located between black arrowheads and the intestinal lumen is marked with a black arrow.