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Rapid activation of nuclear ERK-2 in hippocampal neurons treated with E2 and P4, but not with MPA. Western blots show levels of pERK2 and total ERK2 in cytoplasmic and nuclear fractions from primary hippocampal neurons treated with E2 (A), P4 (B), MPA (C), or combined E2 and progestin (D). Bar graphs represent mean ± SEM; *, P < 0.05 as compared with control; **, P < 0.01 as compared with control; ++, P < 0.01 as compared with E2 alone; n = 4.
