Fig. 2.
SPR analysis of proenzyme formation by native fB. The variant fB proteins were purified from the plasma of donors known to be homozygous for fB32R or fB32Q, representative preparation is indicated (reducing SDS/PAGE gel stained with Coomassie Blue R250). FB was flowed over the surface of the C3b-coated chip at concentrations between 460 and 7 nM in Biacore buffer (10 mM Hepes, pH 7.4/50 mM NaCl/1 mM MgCl2/0.005% surfactant P20). Sensorgrams from fB32R are solid lines and fB32Q are dotted lines; identical concentrations are illustrated for the 2 proteins.