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Binding of the wild-type and mutant FH19–20 proteins to tumor necrosis factor-α-activated mouse glomerular endothelial cells. Serial dilutions of the wild-type and mutant FH19–20 constructs were applied onto the mGEnC-1 cells and binding was detected using enzyme immunoassay. Data are shown in three panels. A, mutants that bound similarly to the wild-type FH19–20; B, mutants that bound stronger than the wild-type.
