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. Author manuscript; available in PMC: 2010 Apr 13.
Published in final edited form as: Cell. 2008 Nov 28;135(5):907–918. doi: 10.1016/j.cell.2008.10.025

Figure 4.

Figure 4

SIRT1 is recruited to chromatin upon DNA damage in an ATM-dependent manner. (A) Western blot analysis of SIRT1 and indicated control proteins in both chromatin-bound and non-chromatin-bound protein fractions from ES cells that were either left untreated or treated with H2O2 or MMS for 1 h. (B and C) Western blot analysis of chromatin-bound SIRT1 in response to H2O2 with or without pretreatment with wortmannin or KU55933 (B), and in the presence or absence of H2AX (C). (D and E) Recruitment of SIRT1 and Rad51 to chromatin in WT or SIRT1-deficient (SIRT1-Δex4) cells in response to H2O2 or IR.