Figure 3.
SIRT1 regulation of SREBP target gene expression during fasting in the mouse liver. qRT–PCR was used to analyze the mRNA levels of SREBP target genes FASN](A), ELOVL6 (B), and SREBP-1c (C) in the mouse liver. The fasting-dependent changes of gene expression are compared between livers of mice subjected to tail-vein injection of adenoviruses programmed to express control shRNA (C; n = 7) and SIRT1 shRNA (shSIRT1; n = 7) (fasted for 20 h). All data are normalized to 18s rRNA expression. Error bars represent SEM. (*) P < 0.05; (**) P < 0.01. (D) Hierarchical clustering of mouse liver transcriptomes with or without SIRT1 knockout. The heat map demonstrates that SREBP target genes are among the most significantly up-regulated genes in SIRT1 knockout livers.