Figure 6.
Sirtuin inhibitors regulate nuclear abundance of SREBP-1a, SREBP-2, and SREBP-1c in primary cells. IMR-90 cells were placed in 1% lipid-depleted serum and proteasome inhibitors and then treated with NAM or sirtinol for 6 h. Cells were stained with antibodies to SREBP-1a (A) or SREBP-2 (B) along with DAPI, and were visualized by immunofluorescence. (C) Hepatocytes from wild-type or SIRT1 LKO mice were stained with antibodies specific for SREBP-1c, and were stained with DAPI to visualize nuclei.