Figure 1. P4-mediated reversal of E2-induced neuroprotection against NMDA toxicity in cultured hippocampal slices.
A. Treatment protocol timeline for hormones and NMDA. Treatment with P4 (10 nM), E2 (10 nM), or NMDA (50 μM) was performed as indicated in the diagram. Vehicle was administered for the length of the experiment (not shown).
B. LDH release in the medium measured 24 h after NDMA treatment. Medium was collected 24 h after initiation of NMDA treatment, and LDH activity was determined as described under Material and Methods. Results are means ± S.E.M of 7 experiments and are expressed as percent of release in NMDA-treated slices. Statistical significance was analyzed by ANOVA followed by Tukey's test for individual comparisons. *p < 0.001 vs. NMDA; † p < 0.001 vs. E2 + NMDA (EN); NS: not significant.