Figure 4. BDNF mediates E2 neuroprotective effects against NMDA toxicity in cultured hippocampal slices.
Cultured hippocampal slices were treated as shown in Figure 1 and TrkB-Fc (0.5 μg/mL) was present during the duration of E2 treatment. Medium was collected 24 h after NMDA treatment and LDH assayed as indicated under Materials and Methods. Results are expressed as percent of LDH release measured in medium of slices treated with NMDA for 3 h and are means ± S.E.M. of 5–6 experiments. Statistical significance was analyzed by ANOVA followed by Tukey's test for individual comparisons. * p < 0.001 as compared to NMDA treated slices.