Figure 5.
LIG4-deficient diploids are delayed in meiosis I. Fractions of cells in sporulating populations that have passed meiosis I, as evidenced by DAPI staining, are shown as a function of time postinduction. Analyses were performed as described in Materials and Methods and averages from two independent experiments are given. The values represent accumulated fractions of cells with two or more DAPI staining bodies in the sporulating populations. Strains are referred to as follows: (LIG4/LIG4) diploid FF18734/FF18984 (▪); (LIG4/lig4) diploids FF18734/PRSY004,2 and PRSY003,1/FF18984 (□); (lig4/lig4) diploid PRSY003,1/PRSY004,2 (♦).