Figure 6.
Immunodetection of Arabidopsis 4HPPD overproduced in transgenic tobacco. Proteins from total extracts (lanes 1) and Percoll- purified chloroplasts (lanes 2) obtained from young leaves of wild-type tobacco (a) (120 μg), transgenic tobacco transformed with ARA9 construct (b) (40 μg), and transgenic tobacco transformed with ARA5 construct (c) (120 μg) were separated on a linear gradient of 3% to 27% (w/v) acrylamide in nondenaturing conditions and analyzed by western blotting using immunopurified polyclonal rabbit antibodies (1:2000 dilution) raised against the purified recombinant carrot 4HPPD (Garcia et al., 1997).