Figure 5. SRT1720 rescues respiratory capacity in mitochondria from wild type mice treated with a high fat diet but not after Sirt1 knockout.
(a) State 3 oxygen consumption by mitochondria isolated from the liver was reduced by HFD. Treatment with SRT1720 in the HFD was protective only in WT mice. (b) Oxygen consumption during state 4 respiration was unchanged by diet or genetic manipulation. (c) The respiratory control ratio was also reduced by HFD and rescued by SRT1720 only in WT mice, as was FCCP-induced respiration (d). n = 4 mice per group; age = 30–32 weeks; diet = 18 weeks. Data are represented as the mean ± SEM. *P < 0.05 from WT SD, #P < 0.05 from KO SD, &P < 0.05 from WT HFD.