Figure 2.
Nucleotide and deduced amino acid sequence of the Arabidopsis HPPDase cDNA pHPPD. The protein sequence is shown in boldface underneath the nucleotide sequence (accession no. AF000228). The nucleotide sequence of the originally identified, truncated expressed sequence tag (accession no. T20952) is indicated by a single underline. Alignments were performed to13 other HPPDase proteins (accession nos. AJ000693, D64004, L38493, U11864, U87257, S69666, M59289, M59429, Z50016, X72389, D29987, M18405, and D13390). Arabidopsis HPPDase amino acid residues showing identity in 9 of the other 13 HPPDase proteins are indicated with shaded boxes. Amino acid residues identical in all 14 HPPDase sequences are denoted with black boxes. The five conserved Tyr and His residues postulated to form the HPPDase ferric iron center are indicated by filled dots. The location of the single 107-bp intron in the HPPDase genomic sequences of Ws and pds1 is denoted by an inverted, filled triangle. The 17-bp deletion in the HPPDase gene in pds1 is denoted by a boldface, italic DNA sequence and two overhead lines. The Ws and pds1 HPPDase gene and protein sequences are identical up to the deletion. The consequence of this mutation at the protein level is indicated in the box below the deletion.