Figure Seven.
Ability of rFH to protect sheep erythrocytes from haemolysis. Sheep erythrocytes were incubated in physiological buffer, with 1.5 μM FH modules 6-8 (negative control), 0.4 μM plasma-purified FH or 0.4 μM rFH, prior to exposure (for 20 min at 37 °C) to human serum that had been depleted of FH. The reaction was quenched and A412 was measured. The results shown were the average (plus or minus standard deviation) of four experiments.