Abstract
The high molecular weight (approximately 30,000) precursor to opioid activity (pro-opiocortin) previously detected in extracts of rat pituitary was digested with trypsin and the resulting peptide mixture was resolved by high-performance reverse-phase chromatography. A peak of opioid activity was eluted at the position of the nonapeptide beta-LPH (61-69), which was also the same fragment obtained by trypsin digestion of betas-lipotropin or beta-endorphin. This identified the protein as a precursor to the endorphins and Met-enkephalin. No activity was detected in the position corresponding to the Leu5 analog of betas-LPH (61-69), thus ruling out the possibility of a beta-lipotropin-like precursor to Leu-enkephalin in pituitary extracts. Pro-opiocortin and beta-lipotropin are present in rat pituitary extracts in comparable amounts, approximately 10 pmol/mg of tissue.
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Selected References
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- Chrétien M., Gilardeau C., Seidah N., Lis M. Purification and partial chemical characterization of human pituitary lipolytic hormone. Can J Biochem. 1976 Sep;54(9):778–782. doi: 10.1139/o76-111. [DOI] [PubMed] [Google Scholar]
- Gráf L., Li C. H. Action of plasmin on ovine beta-lipotropin: revision of the carboxyl terminal sequence. Biochem Biophys Res Commun. 1973 Aug 21;53(4):1304–1309. doi: 10.1016/0006-291x(73)90607-4. [DOI] [PubMed] [Google Scholar]
- Guillemin R., Vargo T., Rossier J., Minick S., Ling N., Rivier C., Vale W., Bloom F. beta-Endorphin and adrenocorticotropin are selected concomitantly by the pituitary gland. Science. 1977 Sep 30;197(4311):1367–1369. doi: 10.1126/science.197601. [DOI] [PubMed] [Google Scholar]
- Lohmar P., Li C. H. Isolation of bovine beta-lipotropic hormone. Biochim Biophys Acta. 1967 Oct 23;147(2):381–383. doi: 10.1016/0005-2795(67)90416-3. [DOI] [PubMed] [Google Scholar]
- Mains R. E., Eipper B. A., Ling N. Common precursor to corticotropins and endorphins. Proc Natl Acad Sci U S A. 1977 Jul;74(7):3014–3018. doi: 10.1073/pnas.74.7.3014. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Rubinstein M., Stein S., Gerber L. D., Udenfriend S. Isolation and characterization of the opioid peptides from rat pituitary: beta-lipotropin. Proc Natl Acad Sci U S A. 1977 Jul;74(7):3052–3055. doi: 10.1073/pnas.74.7.3052. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Rubinstein M., Stein S., Udenfriend S. Isolation and characterization of the opioid peptides from rat pituitary: beta-endorphin. Proc Natl Acad Sci U S A. 1977 Nov;74(11):4969–4972. doi: 10.1073/pnas.74.11.4969. [DOI] [PMC free article] [PubMed] [Google Scholar]